Rethink
Raman Imaging
Nanophoton拉曼显微镜采用线形激光照明和二维CCD技术,一次曝光即可得到400个光谱。通过振镜扫描仪进行激光扫描时,RAMANtouch可在几分钟内,快速、准确地实现数十万像素的拉曼成像——无需电子倍增CCD(EMCCD)。
与传统拉曼显微镜相比,RAMANtouch采用了一种全新的分析方法——这也是它化不可能为可能的原因:在实现最高成像速度的同时,不损失任何光谱质量或空间分辨率。
RAMANtouch具有以下优势:
stay tuned for more !
RAMANtouch使用了振镜,这是一种创新的扫描方法,使激光束可以在物镜下自由扫描目标点——无需移动样品台!不论是精度,还是速度,它都远远超过了传统的“移动台面进行扫描”。
此外,无论您位于视野中心,还是在边缘,激光束都垂直于观察平面入射。这一特点可以实现特殊的测量模式:
以最快的速度和最高的精度,对样品表面进行高光谱和空间分辨率扫描。
通过仅测量拉曼光谱中显示对比度的部分,快速创建测量区域的概览图像。
通过在视野(FOV)上“闪烁”拉曼激光线,创建整个区域的平均光谱。这一特点使得RAMANtouch可以对大面积区域进行超快速测量。
激光线模式
激光点模式
共聚焦光学系统可以在样品内部进行非破坏性拉曼分析。通过这种方式,可以对透明样品创建3D拉曼图像。借助线照明和RAMANtouch的高精度平台,您将能以出色的速度和质量,创建3D拉曼图像,从而深入了解样品的内部结构和成分分布。
视频示例:
在XZ平面上进行深度剖面拉曼成像,可实现非破坏性分析,例如,检查多层薄膜。利用油浸物镜可显著提高空间分辨率,能检测到厚度仅为250纳米的超薄层。
拉曼显微镜为识别有机和无机化合物以及晶体多态性提供了重要洞见。不同于红外显微镜,拉曼显微镜具有亚微米级的空间分辨率,以及在水相环境中分析样品等优势。
| Spatial Resolution | 350 nm in X, 500 nm in Y; 1 µm in Z | |
| Objective Lenses | 5x, 10x, 20x, 50x, 100x | |
| Spectral Resolution | <0.9 cm-1 (depends on grating, up to 3 gratings available) | |
| Stage Details | 30 * 30 * 35 mm XYZ-motorized stage | |
| Calibration | Auto-calibration based on standard lamp and sample | |
| Alignment | Auto-alignment of optical path | |
| Laser Safety | Laser safety class I door with interlock |
When I first encountered a Bruker’s Nanophoton Raman microscope, which was installed for the first time at Analytical center in Chungbuk National University, I was very surprised by the various performances that are different from the previous Raman microscopy. In particular, I was very impressed the function of large-area mapping, which is a disadvantage of most of current Raman microscopy, was solved with the idea of line-illumination.
In our laboratory, Raman spectroscopy was utilized to quantify amino acids in renal cell cultures from an animal model of induced renal dysfunction and to study the distribution of drugs on the cell membrane surface. In addition, the content of illicitly distributed APIs-like compounds was determined by Raman imaging and a semi-quantification method was established.
In addition, the bio-samples that we are mainly researching in our laboratory are very difficult to find the optimum experimental conditions due to the control of the laser power, but the RAMANtouch model can not only adjust a very small power level, but also use the preview function to find the optimal conditions faster than any other Raman product. By using such a powerful Raman microscopy in our laboratory, we have published several number of related papers.
Professor Dr. Yong-Moon Lee, School of Pharmacy, Chungbuk National University, Korea.
The RAMANtouch microscope has made us rethink the limits of application of Raman imaging. The multiplexed acquisition approach brings a significant increase in speed, making it feasible to collect large Raman images instead of few measurement points. The system automation enables our users to utilize measurement time efficiently in overnight runs. This capability adds analytical depth to Raman characterization and facilitates a better understanding of complex research questions and enables big data exploration with machine learning tools.
At Synchrotron Soleil, we facilitate scientific progress by providing access to a high level research infrastructure. Beyond the beamline instrumentation, we also provide additional characterization tools such as offline Infrared or Raman microscopy.
Ferenc Borondis, Beamline Manager & Principal Beamline Scientist at Synchrotron SOLEI, Saint-Aubin, France.
Now, with the pioneering application of the modifed RAMANtouch system to the world’s first 8.5th generation display mass production line, we can achieve groundbreaking real-time defect analysis directly within the production process. By enabling component-level analysis of random defects—responsible for over 90% of yield-loss issues—this technology allows for precise defect identification, helping manufacturers not only trace defect origins and specify affected processes but also prevent these issues in real time.
In the high-stakes realm of display product mass production, advanced inspection technology has become essential for optimizing yield and ensuring top-tier quality control. As these technologies evolve, the need for faster, more precise process feedback has driven demand for real-time monitoring that goes beyond traditional inspection to include advanced measurement and in-depth analysis. Furthermore, In-line customized RAMANtouch’s proactive feedback capabilities offer unprecedented opportunities for continuous process improvement and accident prevention, marking a transformative step forward in display production technology.
Dr. Yong-Woon Lim, Metrology & Inspection Team, Samsung Display, Korea.