MANA 2024

October 23, 2024
12:00 - 1:00 pm EDT

MANA Bruker Lunch Seminar: Florida Lab - Showcasing 4D-Lipidomics™ from the Wild Florida Swamp
October 23, 12:00 - 1:00 pm (EDT)

Join Bruker at the 6^th Annual MANA Conference at the Moffitt Cancer Center in Tampa, Florida for our lunch seminar on October 23 from 12:00 – 1:00 pm.

Agenda:

12:00 – 12:10 pm Introduction
Erica Forsberg, Ph.D., Vice President Metabolomics, Bruker Scientific LLC, San Jose, CA, USA

12:10 – 12:50 pm Florida Lab - Showcasing 4D-Lipidomics™ from the Wild Florida Swamp
Kari Basso, Ph.D., Director of the Mass Spectrometry Research and Education Center, University of Florida, Gainesville, FL, USA

12:50 – 1:00 pm Q&A Session

Abstract

Sample normalization is vital for quantitation. Lipidomic LC-MS/MS is primarily normalized using protein concentration and less often DNA concentration or mass/cell counts; however, there is no widely accepted method nor have lipid-based normalizations been applied. Unlike other lipid quantitation methods, which measure functionalities not representative of most lipids, such as phosphorylation that represents less than 70% of the total lipid abundance, the sulfo-phospho-vanillin assay (SPVA) quantifies unsaturated lipids, representing more than 90% of all lipids.

As the first published SPVA pre-quantitation method to lipidomic LC-MS/MS, similar to a Bradford protein quantitation, biologically sourced standards yielded smaller standard deviations and larger relative concentration differences compared to gravimetric or protein sample normalization methods. We continue investigating the SPVA on true biological lipid extracts, showing the relative lipid concentration effects when lipid or protein concentrations are artificially altered.

Here we will showcase examples of 4D-Lipidomics™ utilizing SPVA normalization on a Bruker timsTOF Pro 2 and MetaboScape^®2024 with a focus on some of the “wilder” samples processed through the Mass Spectrometry Research and Education Center at University of Florida. Recently we have investigated changes in lipid profiles of Grouper tissue from the Gulf of Mexico at different reproductive states and investigated pre- and post-hatching egg yolks of invasive pythons from the Florida everglades.

Kari Basso, Ph.D., Director of the Mass Spectrometry Research and Education Center, University of Florida, Gainesville, FL, USA

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Application Notes:

Brochure:

Brochure - Bio-LP Columns

Poster:

Website:

University of Florida - Department of Chemistry

Published research:

For Research Use Only. Not for use in clinical diagnostic procedures.