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Key Features

  • Close-to-natural environmental conditions for sensitive samples
  • The NEW ready-to-use TruLive3D Dishes facilitate sample mounting
  • Compact, vibration-free and robust design
  • Resolution down to 255 nm in xy
  • Intuitive User Interface

Product Overview

The TruLive3D Imager is optimized for fast 3D multi-sample imaging of delicate live specimens in their native 3D environment.

The optical concept, with dual-sided illumination and single-lens detection from below, enables fast acquisition speed, high-resolution imaging, and minimal shadowing effects.

The system is especially suited for multi-position imaging of small embryos (e.g. mouse), 3D spheroids, oocytes and more, enabling time-lapse, high-throughput imaging experiments.


Large Sample Holder

The sample chamber in the TruLive3D Imager fits a large sample holder (length = 75 mm), which can accommodate tens to hundreds of samples.

Similar to the InVi SPIM, the FEP foil lining the holder serves the dual function of a “curved coverglass” and a physical barrier between the immersion medium and sample medium.

The new ready-to-use TruLive3D Dishes are fully compatible with the TruLive3D Imager. The possibility to line up three disposable dishes extends the capacities of the system to enable the parallel imaging of samples grown under different media conditions.

Click to enlarge
TruLive3D Imager Sample Holder
Click to enlarge
TruLive3D Imager Sample Holder with TruLive3D Dishes

Device Design

The TruLive3D Imager compact, robust and vibration-free design provides maximal stability even during long-term high-throughput experiments.

Tailored to fit your lab bench, this class 1 laser system does not require any air table or vibration-compensation mechanism as all moving components are light-weight and balanced. Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the quality of your sample is affected thanks to the unique and very gentle image-acquisition concept.

InVi SPIM Platform
InVi SPIM Platform
Illumination Detection Effective Magnification Field of View Pixel size Optical Resolution
10x/0.3 NA Nikon 25x/1.1 NA 31.3x
62.5x
420 µm
210 µm
208 nm
104 nm
255 nm

Electronics, Microscope Control and Computer

Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.  

Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.

 

 

Browser-based interface
Browser-based interface

Specifications

Electronics, Microscope Software & Computer

  • Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication
  • Flexible GUI for interactive microscope control and experiment design
  • Computer with 256 GB RAM, Intel dual 8 core CPU
  • High-speed RAID controller for data streaming, 8 × 4 TB local storage in RAID O
  • GeForce GTX 2080 graphics card
  • 10 Gbit/s on-board Ethernet port
  • 1 UHTV 40 inch display