MALDI TOF/TOF

MALDI TOF and TOF/TOF MS

The smarter way to Protein Characterization, Glycoprotein Analysis, QC Applications, Polymer Analysis, Ultra High Throughput Screening and MS Imaging

The analytical advantages of matrix assisted laser desorption ionization TOF MS have been demonstrated for more than 25 years. With inherent advantages including its speed (time to results) and application flexibility, the convenience and effectiveness of MALDI-TOF MS is magnified by virtue of its low sample volume requirements and broad sample content (salts, buffers) amenability. Dramatic increases in the depth of MALDI analyses have been made routine by intuitive  software, and easy automation allows for high throughput solutions.

Bruker’s flagship FLEX series instruments provide reliable results from a market leading technology platform - well-known for outstanding performance, reliability, convenience and innovative design. From rapid screening to comprehensive target characterization, MALDI-TOF MS offers broad utility to meet all laboratory needs. Expert users and beginners can quickly and confidently tap into the analytical power and flexibility of Bruker’s FLEX series MALDI-TOF systems.

Protein or peptide characterization using MALDI-TOF MS workflows quickly provide primary protein sequences of intact protein targets in just minutes. Also higher level protein structure can be examined via the detection and localization of di- and trisulfide bonds. ultrafleXtreme and rapifleX are most suited MALDI- TOF instruments to perform top-down, middle-down, and bottom-up sequencing approaches as well as glyco protein analysis. This includes LC-MALDI based workflows with the spotOn fraction collection and sample preparation station.

For MALDI Imaging rapid data collection speeds, maintenance of critical spatial resolution, and broad dynamic range are mandatory, and, in conjunction with market-leading SCiLS Lab software, are addressed best using ultrafleXtreme or rapifleX instruments. The unbeaten speed of the rapifleX enables ultra high throughput screening and together with our MALDI PharmaPulse 2.2 solution rapid delivery of needed results is ensured.

With a variety of available ionization matrices and the option for analysis in both positive and negative ion modes, MALDI-TOF MS and MS/MS workflows offer critical advantages in the quality control of peptides, oligonucleotides and many classes of polymers. Depending on the analytical depth needed microflex LRF or autoflex maX series in combination with BioPharma Compass software are perfect solutions.

For Research Use Only. Not for Use in Clinical Diagnostic Procedures.