Papilio5D
Papilio5D
The Bruker Papilio5D light-sheet microscope delivers high-sensitivity imaging for organoids and 3D cell cultures with a high degree of flexibility, enabling researchers to adapt the system to specific sample types, study conditions, and experimental designs.
The system’s horizontal lens geometry with dual-view detection and a choice of Gaussian or Bessel beam illumination, combined with support for simultaneous multi-sample and multi-condition imaging, make Papilio5D a flexible solution for achieving deep and high-resolution imaging while maintaining sample integrity — even on challenging samples and in complex, multi-sample, and multi-condition experiments.
To learn more, continue reading, contact us, or see FAQs about this product.
Fast and Photo-Efficient for the Highest Quality Images
Papilio5D is photo-efficient for the most delicate samples, using a horizontal geometry with two views/dual-sided detection and a perpendicular light-sheet. This setup is capable of capturing twice as many photons as single-objective detection setups and enables a more comprehensive 3D view of samples.
Fast dual-view acquisition and fusion deliver high-resolution images. Water-dipping high-NA lenses ensure the light sheet is ultra-thin. Area- and line-mode detection also allow data acquisition specialized for different sample requirements.
Together, these features deliver:
- A high signal-to-noise ratio
- An impressive z resolution
- Optimized depth penetration
Papilio5D can also reach Nyquist sampling, which improves subsequent image processing and enables state-of-the-art deconvolution. Destriping further enhances image quality.
Customizable for Complex Experimental Design
Papilio5D was designed with maximum flexibility in mind. This includes multi-beam and multi-acquisition modes, as well as multi-sample mounting and multi-condition comparison.
Modular illumination beam options
Papilio5D users get to choose the best light beam for their experiment. The choice of Gaussian or Bessel beam illumination, both with a beam width below one micrometer, ensures outstanding image quality and great depth-penetration for a wide variety of objects, including dense organoids or zebrafish larvae and fish.
- Gaussian beams are ideal for small, light-sensitive samples, such as early mouse embryology studies.
- Bessel beams provide multi-direction illumination from a 360° angular range for robust imaging of even optically dense objects.
Multi-acquisition modes
These beams are complemented by two modes of acquisition: area or line mode.
- In area mode, the camera captures the entire field of view at once, making it fast and efficient.
- In line mode, the camera uses a confocal slit-like rolling shutter that synchronizes with the scanned light-sheet. This effectively rejects out-of-focus and scattered light, improving contrast in thick or scattering samples (e.g., organoids).
Multi-sample and multi-condition configurations
The Papilio5D has a generous sample holder that can hold up to three dishes with two separate chambers each, enabling a total of six experimental conditions to be tested at once. Sample health and integrity are further maintained with environmental control that supports long-term imaging (hours to days). Papilio5D provides control over temperature (20 to 34°C), gas concentration (CO₂ and O₂), and humidity.
Optimized for Different Applications
Papilio5D supports a wide range of biological imaging applications, from deep imaging of complex 3D organoids to long-term in vivo studies. Its dual-view detection and multi-sample mounting enable comparative experiments, such as tracking regenerative processes in zebrafish under different treatment conditions.
TO DISCUSS YOUR SPECIFIC APPLICATION, CONTACT US.
In Vivo Time-Lapse After Photomanipulation in Zebrafish
Time-lapse imaging of zebrafish distal tubules after localized photodamaging can provide novel insights into understanding wound healing processes over time. The design of Papilio5D sample mounting within separate chambers enables imaging under different treatment conditions to be studied at the same time. This allows comparative insights into how pharmaceutical components might impact wound healing.
In this example, regeneration of the distal tubule is studied in the embryonic zebrafish kidney using the cdh17:egfp transgenic line after photodamaging with a photomanipulation module.
Select Papilio5D Technical Specifications
| Objective | Tube Lens | Magnification | Pixel Size [nm] | FOV [µm] |
|---|---|---|---|---|
| Detection 2x Nikon CFI APO LWD 25x 1.10 NA | 100 | 12.5x | 520 | 1065 |
| 200 | 25x | 260 | 532 | |
| 300 | 37.5x | 173 | 353 | |
| 400 | 50x | 130 | 266 | |
| Detection 2x Nikon CFI APO LWD 16x 0.8 NA | 100 | 8x | 813 | 1664 |
| 200 | 16x | 406 | 832 | |
| 300 | 24x | 271 | 555 | |
| 400 | 32x | 203 | 416 | |
| DOWNLOAD THE BROCHURE FOR MORE INFORMATION | ||||
Illumination Optics
- Chromatic correction from 440 to 660 nm
- Light-sheet generation by Gaussian or Bessel beam generation and beam scanning
- Flexible light-sheet thickness (2 µm to 6 µm)
- Motorised ND filter set (90%, 10%, 1%) for high flexibility in using low laser power
- Thorlabs 20 W 0.6 NA water immersion objective lens
Detection Optics
- Two Nikon CFI Apo 25x W 1.1 NA water immersion objective lens or two Nikon 16x W 0.8 NA water immersion objective
- Two spectral detection channels, each equipped with a fast filter wheel (10 positions)
- Filters adapted to the selected laser lines
- Three motorised dichroic mirror positions for simultaneous dual channel acquisition (optional)
- Motorised collar ring control (25x)
- Two high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3 (or others)
- Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
- Peak quantum efficiency (QE): 82% @ 560 nm
System Comparison
* COMPATIBLE MODULE
| Papilio5D Dual-view |
MuVi SPIM Multiview |
InVi SPIM Lattice Pro Inverted View |
LCS SPIM Large Cleared Samples |
TruLive3D Imager |
|
|---|---|---|---|---|---|
| Benchtop Design |
✓ | ✓ | ✓ | ✓ | ✓ |
| Configuration |
Horizontal – Dual Detection | Horizontal Multiple-View |
Inverted |
Inverted - Dual Illumination |
Inverted - Dual Illumination |
| # Lenses |
3 lenses (1 IO, 2 DO) | 4 lenses (2 IO, 2 DO) |
2 lenses (1 IO, 1 DO) | 3 lenses (2 IO, 1 DO) | |
| Multi-View |
✓ | ||||
| Live/Fixed Samples |
✓ | ✓ | ✓ | ✓ | |
| Cleared Samples |
✓ | ✓ | |||
| Best Embedding |
Sample holder/three dishes/ two separate chambers | Capillary/FEP tube w/ agarose 3D stage |
FEP foil; glass slides |
Quartz-crystal cuvette | TruLive3D dishes and FEP foil |
| Photomanipulation* |
✓ | ✓ | ✓ | ✓ | |
| Environmental Control* | ✓ | ✓ | ✓ | ✓ | |
| Destriping/Uniform Illumination* | ✓ | ✓ | ✓ (w/ Advanced Illumination Module) | ✓ | ✓ |
All-in-One LuxBundle Software
Intuitive design
Bruker's LuxBundle software saves time and enhances productivity by providing:
- All-in-one, easy-to-use interface for acquisition, viewing, and post-processing
- Fully scriptable microscope control and post-processing via open interface (e.g., Python or any other language), ready for custom "smart" microscopy
- High reproducibility of experiments: all parameters are saved in the metadata and configurations can be saved for future experiments
- Data formats (.tiff, .hdf5, .ims) compatible with common image processing software: Imaris, Aivia, BigDataViewer, Arivis, Fiji, Python, Matlab, Napari
Impressive Image Post Processor
Our dual-view light-sheet microscopes record a sample from different angles/views and generate images composed of multiple tiles. The LuxBundle software ensures high-quality, 360° crisp images of the sample that compensate for absorption and scattering. Features include:
- Multi-color alignment
- Tile stitching of hundreds of tiles for large samples
- Multi-view image fusion and deconvolution
3D Data Viewer
LuxBundle's integrated 3D data viewer allows researchers to inspect the entire dataset directly after acquisition. This gives users control over their data with key capabilities, including:
- The ability to turn tile stitching on or off
- Both raw and post-processed images
- Fast viewing of multi-terabyte data sets
- Flexible options to draw and annotate regions and landmarks
Frequently Asked Questions
Papilio5D is designed for high-sensitivity imaging of organoids, 3D cell cultures, zebrafish larvae, and other optically dense and delicate biological samples.
The sample holder supports three dishes with two chambers each, allowing imaging of six experimental conditions in parallel.
Papilio5D supports Nikon CFI APO LWD 25x 1.10 NA and 16x 0.8 NA objectives. Magnification ranges from 8x to 50x, depending on tube lens configuration (100–400 mm).
Papilio5D supports Gaussian and Bessel beam illumination. Light-sheet thickness is adjustable from 1 µm to 6 µm.
Papilio5D offers area mode for full-field acquisition and line mode with rolling shutter for improved contrast in thick or scattering samples.
Papilio5D provides temperature control (20–34°C), gas regulation (CO₂ and O₂), and humidity management for long-term imaging.
Pixel sizes range from 813 nm to 130 nm. Field of view varies from 1664 µm (at 8x) to 266 µm (at 50x).
Frame rates exceed 80 fps at full frame and reach up to 500 fps with subframe cropping. Peak quantum efficiency is 82% at 560 nm.
Contact us to request more information or discuss your application with a light-sheet microscope expert. You can also download the brochure, request a quote or see a side-by-side comparison of our light-sheet microscope systems.
