Anasys IR3 webslider 2018.065

Life Sciences

Life sciences research is continually achieving new discoveries due to new nanoscale IR spectroscopy. The nanoIR3 provides unrivalled nanoscale FTIR spectroscopy as well as chemical, structural and mechanical property mapping of a broad range of biological materials. Researchers using AFM-IR have generated groundbreaking conclusions in protein secondary structures that are linked to disease formation. Applications include:

  • Cells, proteins, bacteria
  • Tissue, bone and hair
  • Bio-materials and bio-minerals
  • Pharmaceuticals
  • Wood and plants samples
  • Bio polymer films

 

 

AFM-IR has also excelled in providing nanoscale spectroscopy of tissue, bone, wood, bio-materials, bio-minerals, pharmaceuticals, and many other sample types.


Protein Secondary Structure - Single Fiber

Kulik Data

Topographical, chemical, and mechanical analysis of a single collagen fiber, showing amide I absorption. Image courtesy of EPFL, Switzerland.

Protein Structures

Dietler Data

AFM-infrared chemical maps and spectra of Josephin proteins before incubation at 37 deg C. (a) AFM height image. Infrared absorption map at (b) 1700 cm-1 (amide I), (c) 1655 cm-1 (amide I), (d) 1300 cm-1 (amide III). Scale bar, 2mm. (e) Infrared spectra. (f) Average oligomeric infrared spectrum and secondary-structure deconvolution of amide I band Image courtesy of F.S. Ruggeri et al. DOI: 10.1038/ncomms8831


Distribution of Triglycerides in Stratum Corneum

Distribution Of Triglycerides Stratum Corneum

The stratum corneum is the outermost layer of skin and is composed of corneocytes, a dense network of protein, and intercellular lipids. Above: AFM-IR used to map the distribution of the intercellular lipids of the stratum corneum by imaging the absorption band at 1732 cm-1 in comparison to the amide I absorption band at 1650 cm-1.

Chemical Analysis of Hair

HairCross section 2

Light Harvesting Complex II

Light Harvesting Complex

Light-harvesting complex II. (a) Surface topography, (b) mechanical stiffness map and (c) IR absorption pattern of the lipoprotein multibilayer, recorded simultaneously on a 1x1 µm area of the sample, the thickness of which was approximately 1.2 µm. Data contributing to the IR-absorption map were acquired at a wavenumber of 1650 cm-1. Scan resolution: X = 512 points, Y = 256 points; scan rate: 0.4 Hz, 8 co-averages.

Purple Membrane

ResonanceEnhancedAFM IR5nmBiologicalMembrane

Resonance enhanced AFM-IR was used to collect topographical and AFM-IR images at 1660 cm-1 of Halobacterium salinarium membranes. IR spectra identifies the α-helix structure of the membrane protein by the position of the amide I absorption band.


Self-assembled Monolayers

Self Assembled Monolayers

AFM imaging, AFM-IR imaging, and Resonance Enhanced AFM-IR spectroscopy of a monolayer island film of a PEG methyl ether thiol on gold.

Biorenewable Polymer

Biorenewable Polymer2

IR spectra of locally heat treated polyhydroxybutyrate (PHB) reveal variations in crystalline/amorphous content (C-O-C stretches, 1270 cm-1)


Bone

Biominerals absorptionRate

AFM-IR spectra reveal variations in the mineral/protein concentration from the interior to the exterior of an osteon.

 

Biomerals

Pharmaceutical

Pharmaceutical2

AFM-IR is used to study phase separation in active pharmaceutical ingredients and passive carrier materials in drug dispersions. Above:The AFM topography image (left) shows phase separation of polyvinylpyrrolidone (PVP) domains in a dextran matrix which are readily identified by the AFM-IR spectra (right).

Wood

Biomaterials2

Chemical analysis of wood using AFM-IR provides high spatial resolution chemical information of wood composites, preservation treatments, and transgenic wood for biofuels. Above: An AFM topography image (left) of a sectioned wood sample and AFM-IR spectra (right) show variation in the lignin and cellulose ratio between the cell wall and middle lamellae.


Subcellular Chemical Imaging

Life Sciences2

Local absorption spectra of a cancer cell where subtle shifts in amide I and II bands (1648, 1536 cm-1) are noted along with its lipid/triglyceride contents (1732 cm-1)

Surface topography

Surface topography (left) of internal structure of streptomyces bacteria. Image of the lipid vesicle distribution via the triglyceride absorption band (center). AFM-IR spectra of the bacteria (blue) and vesicles (black), identifying carbonyl ester bands in both spectra points (right). Scan size: 20 µm x 10 µm. Deniset-Besseau, et al, Chem. Lett., 5 (4) 654–658 (2014)