Trapped Ion Mobility Spectrometry (TIMS) and Parallel Accumulation Serial Fragmentation (PASEF) for Urine Metabolomic Profiling

This webinar took place on June 4, 2020

Webinar Overview

Human urine is an easily accessible biofluid that has become a promising sample for non-invasive discovery of biomarkers. Urine metabolomics could reveal metabolic differences in response to a specific disease or therapeutic intervention. Urine is mainly free from interfering proteins or lipids but complicated by the presence of metabolic breakdown products from a wide range of by-products derived from diet, drugs, environmental contaminants, endogenous waste and bacterial metabolites, many of which are poorly characterized. Therefore, a robust method for metabolite identification is essential to maximize the potential of utilizing urine as a diagnostic specimen. Here, we evaluate the potential of improving traditional LC-MS workflows for urine metabolomics by implementing trapped ion mobility (TIMS) with parallel accumulation serial fragmentation (PASEF) technology.


Cristina Di Poto
Dynamic Omics, ADPE, BioPharmaceuticals R&D, AstraZeneca, Gaithersburg, MD, US

I am a biologist with a Ph.D. in biochemistry used to carry off more than 10 years of advanced training in high throughput analytical techniques coupled to mass spectrometry, for disease-biomarker discovery. I have extensive knowledge in the metabolomics and proteomics fields from sample preparation of various specimens such as blood derivatives, tissues, and cell lines to MS-data generation and analysis.


For Research Use Only. Not for use in clinical diagnostic procedures.