Featuring a horizontal setup, the MuVi SPIM LS is designed to image large volumes of living objects very fast. The unique 4-axis concept with its two-sided illumination facilitates four orthogonal views of the sample without the need for rotation.
The MuVi SPIM can achieve a resolution down to 300 nm in 3D (at a wavelength of 500 nm), enabling live imaging, free of phototoxic effects.
Two Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lenses project two aligned light sheets from opposing directions on the sample. Detection includes two high numerical aperture Olympus 20x 1.0 NA or Nikon 16x 0.8 NA water immersion objective lenses. An additional magnification changer results in a total magnification that ranges from 12x to 33.3x.
Cutting-edge biological imaging like light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.
The MuVi SPIM can be equipped with environmental control. A Peltier based water cooling/heating system is available in the MuVi SPIM to keep the immersion medium at a homogeneous temperature, while the heated lid prevents condensation. Temperature can be adjusted between 20–37 °C for optimal incubation conditions.
In addition, the MuVi SPIM also provides precise and stable environmental control (i.e. CO2, O2, N2, and humidity). Gas-concentration for the different components ranges between 0–15 % for CO2, 1–21 % for O2 and 20–99 % for H2O (humidity). The gas humidifier offers feedback control for precise regulation.
Browse a selection of applications data from our customers below. Researchers are using MuVi SPIM LS in a variety of ways including studies in embryogenesis and developmental biology, organoids, cell cultures, neurobiology and neurodevelopment, plants, and more.
Transgenic line expressing His2Av-mCherry as fluorescent nuclear reporter. The fruit fly embryo was imaged for almost one complete day (4 × 200 slices every 30 seconds). Imaged on the MuVi SPIM.
European Molecular Biology Laboratory (EMBL)
Zebrafish embryo expressing Histone H2A-GFP imaged every 6 min from late gastrula to 15–17 somite stage. Imaged on the MuVi SPIM.
Caltech, Pasadena, CA, USA
as well as: Course faculty and participants of the 2017 Zebrafish Course
Marine Biological Laboratory (MBL)
Woods Hole, MA, USA
The axon of a neuron in the zebrafish brain was selectively dissected by means of IR laser ablation (MuVi SPIM). Thirty minutes after ablation, four microglia reached the damaged axon.
Image taken from:
de Medeiros, G., Kromm, D., Balazs, B. et al. Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy. Sci Rep 10, 1942(2020). https://doi.org/10.1038/s41598-019-54349-x
Zebrafish eye imaged on the MuVi SPIM.
Anja Machate and Michael Brand
Center for Regenerative Therapies Dresden (CRTD), TU Dresden
||Field of View||Pixel Size||Optical Resolution|
|10x / 0.3 NA||Olympus 20x / 1.0 NA||
|10x / 0.3 NA||Nikon 16x / 0.8 NA||
Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.
Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.