The sample chamber, the illumination, and the detection unit in the octagon are adapted to match the experimental needs in terms of sample size, refractive index, field-of-view, and resolution, adding high flexibility to the system.
The Cleared Sample Module makes the MuVi SPIM geometry suitable for large cleared sample imaging. The exchangeable cleared sample octagon and an overhead sample positioning unit installed on the microscope’s top plate makes it fully compatible with the live sample version of the MuVi SPIM.
The octagon can be easily removed from the microscope for maintenance and cleaning of the sample chamber and the objective lenses that have been exposed to the mounting solution. The sample chamber is compatible with a broad range of solutions used in different clearing protocols. Available in two different sizes (max. volumes of 20 ml and 33 ml), the sample chamber can accommodate large samples such as mouse brains and other complete organs. Sample mounting from above enables easy sample access and optimized travel range suited for various mounting methods (e.g. hook, plate, pin or cuvette).
Flexible illumination and detection configurations leverage the MuVi SPIM CS to meet your application needs and allow imaging of a great variety of samples at high speed and high resolution.
The MuVi SPIM CS can achieve a resolution down to 300nm in 3D (at a wavelength of 500nm), enabling imaging of large optically cleared samples with high resolution.
Two Nikon 10x 0.3 NA air objective lenses project two aligned light sheets from opposing directions on the sample. Detection includes high numerical aperture, multi-immersion objective lenses, with 20x and 10x magnification. An additional magnification changer results in a total magnification that ranges from 7.5x to 30x. You can choose the set of illumination and detection objective lenses according to the experimental needs.
Cutting-edge biological imaging like light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.
Browse a selection of applications data from our customers below. Researchers are using MuVi SPIM CS in a variety of ways including studies.
Cleared mouse lymph node. High endothelial venules (642 nm, red) and autofluorescence (488 nm, green) to visualize surrounding tissue. Imaged on the MuVi SPIM CS.
Courtesy of:
Jens Stein
University of Bern
Bern, Switzerland
BABB-cleared mouse lymph node. High endothelial venules (red) and autofluorescence (green) to visualize surrounding tissue. Imaged on the QuVi SPIM.
Courtesy of:
Jens Stein
University of Bern
Bern, Switzerland
Color-coded depth representation: maximal depth displayed 1.23 mm.
Imaged on the MuVi SPIM CS.
Courtesy of:
Montserrat Coll Lladó
European Molecular Biology Laboratory (EMBL)
Barcelona, Spain
Labeled with anti-tuj1 (green) to mark developing nerves and with anti-desmin (red) to mark differentiating muscles. Tiled image (6 × 5). Imaged on the MuVi SPIM CS.
Courtesy of:
Glenda Comai
Institut Pasteur
Paris, France
Illumination | Detection |
Effective Magnification |
Field of View | Pixel Size | Optical Resolution |
10x / 0.3 NA | Olympus 20x / 1.0 NA | 15.0x 20.0x 30.0x |
890 µm 665 µm 445 µm |
434 nm 325 nm 217 nm |
300nm |
10x / 0.3 NA | Nikon 10x / 0.5 NA | 7.5x 10.0x 15.0x |
1775 µm 1330 µm 890 µm |
867 nm 650 nm 434 nm |
600 nm |
Illumination Optics
Detection Optics
Detection | Depth (z) | Width (y) | Height (x) |
10x | 27mm | 30mm | 40mm |
20x | 27mm | 30mm | 40mm |
Detection | Depth (z) | Width (y) | Height (x) |
10x | 11.6mm | 15mm | 20mm |
20x | 16.4mm | 15mm | 20mm |
Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.
Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.