Designed to image large volumes of living objects very fast

The MuVi SPIM LS is the very first light-sheet microscopy system by LUXENDO.

Featuring a horizontal setup, it is designed to image large volumes of living objects very fast. The unique 4-axis concept with its two-sided illumination facilitates four orthogonal views of the sample without the need for rotation.

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MuVi SPIM Light-Sheet Microscope

Illumination and Detection

The MuVi SPIM can achieve a resolution down to 300 nm in 3D (at a wavelength of 500 nm), enabling live imaging, free of phototoxic effects.

Two Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lenses project two aligned light sheets from opposing directions on the sample. Detection includes two high numerical aperture Olympus 20x 1.0 NA or Nikon 16x 0.8 NA water immersion objective lenses. An additional magnification changer results in a total magnification that ranges from 12x to 33.3x.

Illumination Detection Effective Magnification Field of View Pixel size Optical Resolution
10x/0.3 NA Olympus 20x/1.0 NA 16.7x
22.2x
33.3x
800 µm
600 µm
400 µm
389 nm
293 nm
195 nm
300 nm
10x/0.3 NA Nikon 16x/0.8 NA 12.0x
16.0x
24.0x
542 µm
406 µm
271 µm
542 nm
406 nm
271 nm
375 nm
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Muvi SPIM LS – Multiview illumination and detection concept
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MuVi SPIM LS – Four orthogonal views of the specimen

Illumination Optics

  • Chromatic correction from 440 to 660 nm
  • Light-sheet generation by beam scanning
  • Flexible light-sheet thickness (2 µm to 8 µm)
  • 2 Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens
  • 2 identical illumination arms

 

Detection Optics

  • Two Olympus 20x 1.0 NA or two Nikon 16x 0.8 NA water immersion objective lenses
  • 2 identical detection arms, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
  • Filters adapted to the selected laser lines
  • 2 high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
  • Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
  • Peak quantum efficiency (QE): 82% @ 560 nm