Mass Spectrometry (MS) based proteomics has become a crucial technique for the majority of proteome studies. As techniques and instruments have improved, comprehensive proteome studies now are beginning to capture accurate qualitative and compositional (post-translational modification) information at an unprecedented scale. The development of more sensitive instruments is a constant driving focus in the industry, with the aim of achieving high precision and accuracy of identifications and quantifications.
In investigating the capabilities of the new timsTOF Pro from Bruker, we discuss various strategies to improve sample preparation, chromatographic resolution and instrument acquisition modes. This will include recent advancements in high-throughput sample techniques for label free quantitation (LFQ) and phosphopeptide enrichment, developments in ultra-short nanoUHPLC columns (facilitating in excess of 50 runs per day per instrument), and instrument acquisition modes including PASEF and the data independent acquisition (DIA) modes on the timsTOF Pro. Finally, we will examine the value of having true accurate collisional cross-sectional (CSS) values in future proteomics workflows, and touch on the future directions of the field as we begin to integrate deep learning approaches and proteomics into inter-omics research.
Associate Professor Walter and Eliza Hall Institute (WEHI)
For Research Use Only. Not for use in clinical diagnostic procedures.