Luxendo Light-Sheet Microscopes

MuVi SPIM LS

The multiview light-sheet microscope for live samples
MuVi SPIM LS (Live Sample)

Highlights

MuVi SPIM LS Imaging Live Samples with Speed

Featuring a horizontal setup, the MuVi SPIM LS is designed to image large volumes of living objects very fast. The unique 4-axis concept with its two-sided illumination facilitates four orthogonal views of the sample without the need for rotation.

Features

Illumination and Detection

The MuVi SPIM can achieve a resolution down to 300 nm in 3D (at a wavelength of 500 nm), enabling live imaging, free of phototoxic effects.

Two Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lenses project two aligned light sheets from opposing directions on the sample. Detection includes two high numerical aperture Olympus 20x 1.0 NA or Nikon 16x 0.8 NA water immersion objective lenses. An additional magnification changer results in a total magnification that ranges from 12x to 33.3x.

Lux DATA - A Comprehensive Data Processing and Storage Solution

Cutting-edge biological imaging like light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.

Environmental Control

The MuVi SPIM can be equipped with environmental control. A Peltier based water cooling/heating system is available in the MuVi SPIM to keep the immersion medium at a homogeneous temperature, while the heated lid prevents condensation. Temperature can be adjusted between 20–37 °C for optimal incubation conditions.

In addition, the MuVi SPIM also provides precise and stable environmental control (i.e. CO2, O2, N2, and humidity). Gas-concentration for the different components ranges between 0–15 % for CO2, 1–21 % for O2 and 20–99 % for H2O (humidity). The gas humidifier offers feedback control for precise regulation.

Applications

Live Sample Applications

Browse a selection of applications data from our customers below. Researchers are using MuVi SPIM LS in a variety of ways including studies in embryogenesis and developmental biology, organoids, cell cultures, neurobiology and neurodevelopment, plants, and more.

Drosophila Embryo Development

Transgenic line expressing His2Av-mCherry as fluorescent nuclear reporter. The fruit fly embryo was imaged for almost one complete day (4 × 200 slices every 30 seconds). Imaged on the MuVi SPIM.

Courtesy of:
Cilia Smits and Stanislav Y. Shvartsman
Department of Molecular Biology
Princeton University, NJ, USA

 

Zebrafish Embryonic Development

Zebrafish embryo expressing Histone H2A-GFP imaged every 6 min from late gastrula to 15–17 somite stage. Imaged on the MuVi SPIM.

Courtesy of:
Andres Collazo
Caltech, Pasadena, CA, USA
as well as: Course faculty and participants of the 2017 Zebrafish Course
Marine Biological Laboratory (MBL)
Woods Hole, MA, USA

Microglia Response to Axonal Damage

The axon of a neuron in the zebrafish brain was selectively dissected by means of IR laser ablation (MuVi SPIM). Thirty minutes after ablation, four microglia reached the damaged axon.

Image taken from:
de Medeiros, G., Kromm, D., Balazs, B. et al. Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy. Sci Rep 10, 1942(2020). https://doi.org/10.1038/s41598-019-54349-x

 

Zebrafish Eye

Zebrafish eye imaged on the MuVi SPIM.

Courtesy of:
Anja Machate and Michael Brand
Center for Regenerative Therapies Dresden (CRTD), TU Dresden
Dresden, Germany

Specifications

Specifications

Illumination Detection
Effective Magnification
Field of View Pixel Size Optical Resolution
10x / 0.3 NA Olympus 20x / 1.0 NA

16.7x

22.2x

33.3x

800 µm

600 µm

400 µm

389 nm

293 nm

195 nm

300nm
10x / 0.3 NA Nikon 16x / 0.8 NA

12.0x

16.0x

24.0x

1110 µm

832 µm

555 µm

542 nm

406 nm

271 nm

375 nm

Illumination Optics

  • Chromatic correction from 440 to 660 nm
  • Light-sheet generation by beam scanning
  • Flexible light-sheet thickness (2 µm to 8 µm)
  • 2 Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens
  • 2 identical illumination arms

Detection Optics

  • Two Olympus 20x 1.0 NA or two Nikon 16x 0.8 NA water immersion objective lenses
  • 2 identical detection arms, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
  • Filters adapted to the selected laser lines
  • 2 high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
  • Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5 µm × 6.5 µm size) and up to 500 fps at subframe cropping
  • Peak quantum efficiency (QE): 82% @ 560 nm

Software

Software

Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.

Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.

Electronics, Microscope Software and Computer

  • Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication
  • Flexible GUI for interactive microscope control and experiment design
  • Computer with 256 GB RAM, Intel dual 8 core CPU
  • High-speed RAID controller for data streaming, 8×4 TB local storage in RAID 0
  • GeForce RTX 3070 graphics card
  • 2 x 10 Gbit/s, 2 x 1 Gbit/s, optional 2 x  optical port 
  • 43 inch 4k UHD display

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