TimsTOF PASEF 1

Advancing Proteomics Research with the timsTOF Pro

This webinar took place on May 07th 2019

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Advancing proteomics research with the timsTOF Pro"

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Overview

Mass Spectrometry (MS) based proteomics has become a crucial technique for the majority of proteome studies. As techniques and instruments have improved, comprehensive proteome studies now are beginning to capture accurate qualitative and compositional (post-translational modification) information at an unprecedented scale. The development of more sensitive instruments is a constant driving focus in the industry, with the aim of achieving high precision and accuracy of identifications and quantifications. 

In investigating the capabilities of the new timsTOF Pro from Bruker, we discuss various strategies to improve sample preparation, chromatographic resolution and instrument acquisition modes. This will include recent advancements in high-throughput sample techniques for label free quantitation (LFQ) and phosphopeptide enrichment, developments in ultra-short nanoUHPLC columns (facilitating in excess of 50 runs per day per instrument), and instrument acquisition modes including PASEF and the data independent acquisition (DIA) modes on the timsTOF Pro. Finally, we will examine the value of having true accurate collisional cross-sectional (CSS) values in future proteomics workflows, and touch on the future directions of the field as we begin to integrate deep learning approaches and proteomics into inter-omics research.

Key Learnings

  • Sample preparation techniques that take advantage of ultra-sensitive MS instruments
  • NanoUHPLC improvements to match the speed of timsTOF Pro acquisition
  • Acquisition modes on the timsTOF Pro – PASEF and DIA
  • The value of true CCS values in proteomics researc

Who should attend?

  • Scientists working in the field of proteomics

Speaker

Andrew Webb
Andrew Webb
Associate Professor Walter and Eliza Hall Institute (WEHI)
Andrew Webb has over 15 years of experience in the life sciences and proteomics fields. Andrew joined the Walter and Eliza Hall Institute (WEHI) in 2011 to develop the institutes mass spectrometry and proteomics capacity. Prior to joining WEHI, Andrew was trained in a variety of areas including Biochemistry, Immunology and Virology with a focus on interfacing high end technology with medical research.