Ex Vivo MicroangioCT: Advances in Microvascular Imaging
Although microCT provides volumetric data at a near-microscopic resolution, the technique’s inherent X-ray attenuation means the vasculature cannot be discriminated from the surrounding soft tissues.
In this webinar, Dr. Ruslan Hlushchuk (Universität Bern, Institute of Anatomy) and Dr. Kjell Laperre (Bruker Biospin’s Pre-Clinical Imaging Division) will introduce a new radiopaque contrast agent called μAngiofil that can be used to overcome this challenge.
For ex vivo angiography, it provides microvascular imaging down to the fine-capillary level, without requiring prior tissue maceration or bone removal/decalcification. Other advantages are that the sample can be used for subsequent correlative morphology and quantitative analysis can be performed, without requiring extensive post-processing.
What to expect
Ruslan will describe the microCT-based visualization that can be achieved with the polymerizing contrast agent μAngiofil. He will discuss the perfusion procedure, sample harvesting and handling involved. Bruker Biospin’s Skyscan 1272 will be introduced, as well as the higher energy Skyscan 1273 and 2214 that enable the imaging of denser samples.
- The perfusion procedure/protocol for μAngiofil – visual control of the perfusion quality, sample harvesting and tendering.
- The non-destructive imaging of microvasculature down to the finest capillaries, as well as in the presence of bone tissue and/or metal implants.
- The potential for correlative morphology (facilitated by the autofluorescence of the μAngiofil).
- Bruker’s BioSpin’s high resolution desktop micro-CT, Skyscan 1272.
Who should attend?
This webinar will interest customers and potential customers, facility managers, people from contract research organizations, as well as preclinical researchers and people in basic research who specialize in cardiovascular- neurovascular- and angiogenesis-related fields. It would also appeal to implantology researchers.