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Trapped Ion Mobility Spectrometry (TIMS) and Parallel Accumulation Serial Fragmentation (PASEF) for Urine Metabolomic Profiling

This webinar took place on June 04th 2020

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Overview

Duration: 19 minutes

Webinar Summary

Human urine is an easily accessible biofluid that has become a promising sample for non-invasive discovery of biomarkers. Urine metabolomics could reveal metabolic differences in response to a specific disease or therapeutic intervention. Urine is mainly free from interfering proteins or lipids but complicated by the presence of metabolic breakdown products from a wide range of by-products derived from diet, drugs, environmental contaminants, endogenous waste and bacterial metabolites, many of which are poorly characterized. Therefore, a robust method for metabolite identification is essential to maximize the potential of utilizing urine as a diagnostic specimen. Here, we evaluate the potential of improving traditional LC-MS workflows for urine metabolomics by implementing trapped ion mobility (TIMS) with parallel accumulation serial fragmentation (PASEF) technology.

Speaker

Cristina Di Poto
Cristina Di Poto
Dynamic Omics, ADPE, BioPharmaceuticals R&D, AstraZeneca, Gaithersburg, MD, US
I am a biologist with a Ph.D. in biochemistry used to carry off more than 10 years of advanced training in high throughput analytical techniques coupled to mass spectrometry, for disease-biomarker discovery. I have extensive knowledge in the metabolomics and proteomics fields from sample preparation of various specimens such as blood derivatives, tissues, and cell lines to MS-data generation and analysis.