It is perfectly suited for a great variety of samples and applications ranging from cell culture to whole embryos (e.g. Drosophila and Zebrafish). For additional customization options, see the InVi SPIM Lattice Pro.
The InVi SPIM can achieve a resolution down to 255 nm in xy, enabling subcellular resolution in living samples free of phototoxic effects.
A Nikon CFI Plan Fluor 10x W 0.3 NA water immersion objective lens projects the light-sheet on the sample. Detection includes a high numerical aperture Nikon CFI Apo 25x W 1.1 NA water immersion objective lens. An additional magnification changer results in 31.3x and 62.5x total magnification for field view and sampling adjustment according to your experimental needs.
Cutting-edge biological imaging like light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.
Browse a selection of applications data from our customers below. Researchers are using InVi SPIM in a variety of ways including studies in embryogenesis and developmental biology, organoids, cell cultures, neurobiology and neurodevelopment, plants, and more.
Characterization and imaging of stochastic tumorigenesis in mammary organoids. Imaged on the InVi SPIM.
A. Alladin, L. Chaible, L. Garcia del Valle, S. Reither Sabine, M. Loeschinger, M. Wachsmuth, J.K. Hériché, C. Tischer, M, Jechlinger. Tracking cells in epithelial acini by light-sheet microscopy reveals proximity effects in breast cancer initiation. eLife 2020;9:e54066 doi: 10.7554/eLife.54066
Spheroid stained with anti-GFAP (Alexa 488) to label astrocytes and anti-Neurofilament200 (Alexa555) to label neurons. Imaged on the InVi SPIM.
AG Leist, University of Konstanz
Mitosis in HeLa cells stained for histone 2B-mCherry (magenta), GFP-tubulin (green) and GFP-tubulin (white, deconvolved).
Imaged on the InVi SPIM Lattice Pro.
Visualization: Imaris (Bitplane).
European Molecualr Biology Laboratory (EMBL)
Sample: HeLa cells (Neumann et al., Nature. 2010 Apr 1;464(7289):721-7)
Left: Mouse preimplantation embryos expressing H2B-mCherry. Nuclei tracking from one-cell stage to blastocyst.
Right: Mouse oocytes expressing CENPC-EGFP and H2B-mCherry for kinetochore tracking.
Imaged on the InVi SPIM.
Petr Strnad, et al. (2016). Inverted light-sheet microscope for imaging mouse pre-implantation development. Nature Methods 13, 139-145
Transgenic Arabidopsis root expressing a membrane marker. Imaged with the InVi SPIM.
COS, University of Heidelberg
Protein dynamics in C. elegans skeleton. Imaged on the InVi SPIM.
Dr. Chai Yongping and Dr. Ou Guangshuo
||Field of View||Pixel Size||Optical Resolution|
|10x / 0.3 NA||Nikon 25x / 1.1 NA||