Luxendo Light-Sheet Microscopes

QuVi SPIM

The quantitative view light-sheet microscope
QuVi SPIM (Quantitative View Light-Sheet Microscope)

Основные моменты

QuVi SPIM

The QuVi SPIM is a highly versatile upright, dual view and dual color light-sheet microscope for 3D imaging of living, fixed and cleared samples. Based on our robust, stable system platform, it combines dual-view acquisition with high precision, long travel range stages. The QuVi SPIM is optimized for long-term, high-throughput imaging at subcellular isotropic resolution. It features an easy sample mounting and an intuitive software and hardware concept.

Dual View / Dual Acquisition
Two different configurations available: Simultaneous dual-channel acquisition (QuVi) allows for correlative microscopy while the simultaneous dual view acquisition (expert QuVi) allows for isotropic detection of rapid biological processes.
Optimized Stability
Highly stable imaging and incubation conditions for long-term live imaging
User-friendly
Software and Hardware
All microscope functions are controlled by easy-to-use software. Sample mounting and switching from live to cleared samples is effortless through quick-load function and plug-&-play hardware.

Особенности

Simultaneous Dual-View or Dual-Channel Acquisition

The QuVi SPIM combines dual views with dual detection channels to enable large-scale 3D imaging of life specimens and cleared samples. The system enables fast and simple transition between living and cleared tissue imaging.

Typical applications of the microscope include living or fixed and cleared brain slices, long-term imaging of 3D cell culture models (spheroids, organoids, tumoroids), imaging of conventional cell culture in high throughput, and even functional (e.g. calcium) imaging.

 

Optimized Stability

The QuVi SPIM can be equipped with environmental control. Temperature can be adjusted between 20–37°C for optimal incubation conditions.

In addition, the QuVi SPIM also provides precise and stable environmental control (i.e. CO2, O2, N2, and humidity). Gas-concentration for the different components ranges between 0–15 % for CO2, 1–21 % for O2, and 20–99 % for H2O (humidity). The gas humidifier offers feedback control for precise regulation.

 

User-Friendly Software and Hardware

Hardware

Its novel stage design allows using SBS-format plates and provides an easy sample accessibility by means of its quick load feature. Since the objective lenses are exchangeable, a wide variety of samples of different sizes and preparations can be imaged.

Software

Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.

Many Design Features Make the QuVi Excell

Upright, Dual View and Dual Color

The QuVi SPIM combines easy accessibility with high throughput and well-controlled sample conditions, e.g. to perform screening experiments or biophysical methods such as FLIM and FCS.

Typical applications of the microscope include living or fixed and cleared brain slices, long-term imaging of 3D cell culture models (spheroids, organoids, tumoroids), imaging of conventional cell culture in high throughput, and even functional (e.g. calcium) imaging.

Illumination and Detection

The QuVi SPIM can achieve a resolution down to 340 nm in 3D, enabling live imaging, free of phototoxic effects.

The QuVi features a dual-side alternating illumination/detection concept and provides two orthogonal views of the sample.

The illumination optics comprise light-sheet generation by scanning a beam after passing a beam-shaper unit for length and diameter adjustment. Two Nikon 40x 0.8 NA water immersion objective lenses alternatingly project two orthogonal light-sheets on the sample. The expandable and upgradable illumination setup allows a range of up to six lasers. The alignment-free light-source offers 40mW power output for each line. For detection, the same objective lenses are used.

The two spectral detection channels equipped with 10 position high-speed filter wheels and a dichroic mirror changer enable simultaneous dual channel imaging. In addition, more than two channels can be acquired sequentially. The robust sCMOS Orca Flash 4.0 V3 cameras from Hamamatsu are well-suited for experiments that require high detection efficiency, quantification and speed.

Device Design

The QuVi SPIM's compact, vibration-free and robust design provides maximal stability during your long-term experiments.

Tailored to fit your lab bench, this class 1 laser system does not require any air table or vibration-compensation mechanism as all moving components are light-weight and balanced. Maximal stability of focus and thermal conditions are also guaranteed. The proprietary piezo-crawler stages ensure longevity and precision for a permanently accurate specimen positioning. Neither the images nor the natural growth behavior of your sample are affected by the gentle image recording.

Lux DATA - A Comprehensive Data Processing and Storage Solution

Cutting-edge biological imaging like light-sheet microscopy, but also super-resolution or two-photon imaging, generates a vast amount of data required to provide researchers with all the relevant information about their samples. However, storage, transfer, and processing of the data remain a challenge.

Применения

QuVi Applications

Researchers are using the QuVi SPIM in a variety of ways including studies in embryogenesis and developmental biology, organoids, cell cultures, neurobiology and neurodevelopment, plants, and more. Browse a selection of applications data from our customers below or visit our more extensive galleries from all Bruker light-sheet solutions.

Cleared Mouse Lymph Node

BABB-cleared mouse lymph node. High endothelial venules (red) and autofluorescence (green) to visualize surrounding tissue. Imaged on the QuVi SPIM.

Courtesy of:
Jens Stein
University of Bern
Bern, Switzerland

3D Cell Culture

3D culture system of human primary cells imaged on the QuVi SPIM.

Courtesy of:
Yassen Abbas
Turco Lab, University of Cambridge
Cambridge, UK

 

C. elegans Lineage Tracing

The histone marker (H2A::mCherry, purple) and the membrane marker (cnd-1::GFP, green) were used for lineage tracing in C. elegans. Imaged on the QuVi SPIM.

Courtesy of:
Zhirong Bao
The Zhirong Bao Lab
Memorial Sloan Kettering Cancer Center (MSKCC)
Ney York, USA

Microglia Movement

Microglia movement in zebrafish. The vascular system is labeled with a cyan marker and microglia with a yellow one. Imaged on the QuVi SPIM at 2 FPS for 20 min. Two orthogonal views fused and max. project.

Courtesy of:
N. Norlin, F. peri
European Molecular Biology Laboratory (EMBL)
Heidelberg, Germany

 

Спецификации

Specifications

Illumination Detection
Effective Magnification
Field of View Pixel Size Optical Resolution
40x / 0.8 NA 40x / 0.8 NA 40.0x
330 µm

160 nm

340nm

Illumination Optics

  • Chromatic correction from 440 to 660 nm
  • Light-sheet generation by beam scanning
  • Flexible light-sheet thickness (2 µm to 6 µm)
  • Two Nikon CFI Apo 40x W 0.8 NA NIR water immersion objective lenses

 

 

Detection Optics

  • Two Nikon CFI Apo 40x W 0.8 NA NIR water immersion objective lenses
  • 3rd objective lens CFI Plan Achromat 4x 0.1 Air
  • Dual-side alternating illumination/detection concept
  • 2 spectral detection channels, each equipped with a fast filter wheel (10 positions and 50 ms switching time between adjacent positions)
  • Filters adapted to the selected laser lines
  • 2 high-speed sCMOS cameras Hamamatsu Orca Flash 4.0 V3
  • Maximum frame rate >80 fps at full frame (2048 × 2048 pixels of 6.5µm × 6.5 µm size) and up to 500 fps at subframe cropping
  • Peak quantum efficiency (QE): 82% @ 560 nm
  • 3rd detection path equipped with a Grasshopper3 5.0MP camera for transmitted light detection

Stage

  • High-precision XYZ stage
  • Unique quick-load feature, SBS plate compatible
  • Easy access from above for sample mounting, injections, etc.
  • Environmental control unit is optinally available, featuring precise control of temperature (range 20–37°C), humidity, O2 and CO2

Laser Combiner

  • Maximum of 6 laser wavelengths (405, 445, 488, 515, 532, 561, 594, 642, and 685 nm with 40 mW each before fiber)
  • Fast modulation (MHz range) and high extinction ratio

Программное обеспечение

Software

Luxendo's intuitive user interface offers a simple setup and execution of multidimensional experiments, while real-time control is handled by an embedded controller to ensure microsecond-precision timing independent of the PC’s performance fluctuations.

Precise timing control of all connected devices is a prerequisite for reliable experimental outcomes. Full control of data streaming to storage as well as GPU-supported image processing further complements the overall performance.

Electronics, Microscope Software and Computer

  • Embedded microscope software with an open communication interface: documented API, TCP/IP-based communication
  • Flexible GUI for interactive microscope control and experiment design
  • Computer with 128 GB RAM, Intel dual 8 core CPU
  • High-speed RAID controller for data streaming, 8 × 4 TB local storage in RAID O
  • GeForce RTX 2080 graphics card
  • 10 Gbit/s on-board Ethernet port
  • 1 UHTV 40 inch display

Вебинары

Специалист

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