Light-Sheet Microscopes
For fast, 3D imaging of live or cleared-tissue samples
See Biology Across All Scales
With a strong focus on applications, our light-sheet solutions are easy to use and enable scientists around the globe to excel in their research. The SPIM technology avoids sample phototoxicity by sequentially illuminating a stack of thin slices of the organism, allowing scientists to observe living organisms for extended periods of time without photodamage.
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Browse Our Light-Sheet Microscopy Library
Browse Light-Sheet Microscopy Articles
| Year | Journal | Title | Author(s) | Imaging Platform | Subject | Methodology |
|---|---|---|---|---|---|---|
| 2023 | The EMBO Journal | Embryo-uterine interaction coordinates mouse embryogenesis during implantation | V Bondarenko, M Nikolaev, D Kromm,(..)Takashi Hiiragi | InVi SPIM | Pre-implantaion development, Morphogenesis, Patterning | Mouse, Ex-vivo, Live imaging, Laser ablation |
| 2023 | Journal of Neuroscience | A Cellular Ground Truth to Develop MRI Signatures in Glioma Models by Correlative Light Sheet Microscopy and Atlas-Based Coregistration | K Schregel, L Heinz, J Hunger, C Pan, (..) Michael O. Breckwoldt | |||
| 2023 | Clinical and Translational Medicine | Basal cell adhesion molecule promotes metastasis‐associated processes in ovarian cancer | S Sivakumar, S Lieber, D Librizzi, (..) Sabine Müller-Brüsselbach, Rolf Müller | LCS SPIM | ovarian cancer, metastasis | Basal cell adhesion molecule (BCAM) is a laminin α5 (LAMA5), Biochemical, omics-based and real-time cell assays |
| 2023 | Advanced Science | The Role of PRRC2B in Cerebral Vascular Remodeling Under Acute Hypoxia in Mice | S Li, W Hu, S Gong, P Zhang, J Cheng,(..) Zengqiang Yuan | MuVi SPIM | hypoxia | mouse, high altitude exposure, cerebral vasculature, RNA immunoprcipitation-seq, and transcriptomic co-analysis |
| 2022 | Nature | Periodic formation of epithelial somites from human pluripotent stem cells | M Sanaki-Matsumiya, M Matsuda, N Gritti… Miki Ebisuya | MuVi SPIM | embryonic development, somitogenesis, segmentation clock | Human iPSC cultures, human somitoids, human somitoids, HCR, IHC, timelapses, human somitoids, morphometry |
| 2022 | National Acad Sciences | Dynamics of Drosophila endoderm specification | SE Keenan, M Avdeeva, L Yang… Stanislav Y. Shvartsman | MuVi SPIM | Drosophila 4D imaging, developmental patterning, gene regulatory network | drosophila, Time-lapse microscopy, single-cell, modelling, image analysis |
| 2022 | Mammary Stem Cells: Methods | Modification of Single Cells Within Mouse Mammary Gland Derived Acini via Viral Transduction | LG Del Valle, MG Montero, M Jechlinger | InVi light-sheet | organoid culture optimization | mouse, organoid cultures, primary donor tissue, 3D acini |
| 2022 | Nature | Embryo-scale epithelial buckling forms a propagating furrow that initiates gastrulation | J Fierling, A John, B Delorme, A Torzynski… Matteo Rauzi | biomechanics, tissue buckling, mesoderm internalization | Drosophila , actomyosin contraction, computational models, in toto embryo image analysis and manipulation | |
| 2022 | Cell | An ex vivo system to study cellular dynamics underlying mouse peri-implantation development | Takafumi Ichikawa, Hui Ting Zhang, Laura Panavaite, ... Takashi Hiiragi | peri-implantation embryo development, mechano-chemical interactions | mouse, 3D ex vivo culture | |
| 2022 | https://link.springer.com/protocol/10.1007/978-1-0716-2659-7_7 | |||||
| 2022 | The Lancet | Müller glia fused with adult stem cells undergo neural differentiation in human retinal models | SÀ Bonilla-Pons, S Nakagawa, EG Bahima… Maria Pia Cosma | MuVi SPIM | retina organoids, Müller glia, regeneration | organotypic cultures of human retina, preparations of dissociated cells, microinjection system, qRT-PCR, immunofluorescence, Flow cytometry |
| 2022 | Genome Biology | Parental genomes segregate into distinct blastomeres during multipolar zygotic divisions leading to mixoploid and chimeric blastocysts | T De Coster, H Masset, O Tšuiko, M Catteeuw, Y Zhao… Joris Robert Vermeesch | InVi SPIM | genome segregation, zygotic division | Bovine embryo in vitro production, imaging, genome analysis, Nuclear staining, human biopsies |
| 2022 | iScience | Discovery of a novel SHIP1 agonist that promotes degradation of lipid-laden phagocytic cargo by microglia | C Pedicone, S Fernandes, A Matera, ST Meyer, S Loh…William G. Kerr | InVi SPIM | degradation of Synaptosomes, phagocytic degradation, microglia | artificial intelligence, mice, computational method, CRISPR/Cas9, immunofluorescence, flow cytometry |
| 2022 | STEM CELLS AND REGENERATION | Rapid and robust directed differentiation of mouse epiblast stem cells into definitive endoderm and forebrain organoids | D Medina-Cano, EK Corrigan, RA Glenn… Thomas Vierbuchen | TrueLive3D Imager | developmental biology, organoids | mouse, pluripotent stem cells, Wnt pathway inhibitors, definitive endoderm and neural organoids,differentiation models |
| 2022 | Science | Parallel evolution of a splicing program controlling neuronal excitability in flies and mammals | A Torres-Méndez, S Pop, S Bonnal, I Almudi, .. Manuel Irimia | MuVi SPIM | phylogeny, evolution, neuronal transcriptomic complexity | neuronal imaging experiments and cell type–specific rescues |
| 2022 | EMBO Reports | PLETHORA‐WOX5 interaction and subnuclear localization control Arabidopsis root stem cell maintenance | RC Burkart, VI Strotmann, GK Kirschner, A Akinci…Yvonne Stahl | MuVi SPIM | stem cell niche | Arabidopsis, lateral root primordium, cloning, staining, Nicotiana benthamiana infiltration, SCN staining, RNA staining, imaging, image analysis |
| 2022 | Developmental Cell | Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster | I Miguel-Escalada, MA Maestro, D Balboa, A Elek… Jorge Ferrer | MuVi SPIM | polygenic disease, pancreas agenesis, enhancer cluster | immunohistochemistry, RT-PCR, Flow cytometry, Seq analysis, ChIP-Seq analysis |
| 2022 | Cell Reports | The neurogenic fate of the hindbrain boundaries relies on Notch3-dependent asymmetric cell divisions | CF Hevia, C Engel-Pizcueta, F Udina, C Pujades | MuVi SPIM | hindbrain morphogenesis, Notch signalling, stem cells, neurogenesis | zebrafish, CRISPR-Cas9, ISH, pharmacological treatments, immunohistochemistry, imaging, image analysis |
| 2021 | eLife | Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development | L Zilova, V Weinhardt, T Tavhelidse, C Schlagheck… Joachim Wittbrodt | MuVi SPIM | morphogenesis and differentiation | medaka and zebrafish, embryonic pluripotent stem cells, fluorescent labeling, quantification |
| 2021 | Plant & Cell Physiology | Integration of Cell Growth and Asymmetric Division during Lateral Root Initiation in Arabidopsis thaliana | LM Schütz, M Louveaux, A Vilches Barro…Alexis Maizel | MuVi SPIM | Lateral root formation, periclinal division | Arabidopsis thaliana, four-dimensional reconstruction, lineage tracking, segmentation |
| 2021 | Biophysical Journal | Pinching and pushing: fold formation in the Drosophila dorsal epidermis | V Velagala, JJ Zartman | MuVi SPIM | planar epithelial sheets, morphogenesis, Hedgehog signalling | Drosophila, immunostaining, image processing, image analysis |
| 2021 | Valve-based consecutive bioprinting method for multimaterial tissue-like constructs with controllable interfaces | |||||
| 2021 | BMC Dev Biol | The migratory pathways of the cells that form the endocardium, dorsal aortae, and head vasculature in the mouse embryo | C Collart, A Ciccarelli…J. C. Smith | MuVi SPIM | Vasculogenesis, embryogenesis | mouse embryo, single-cell transcriptomics, imaging, OPT |
| 2021 | Developmental Cell | A two-tier junctional mechanism drives simultaneous tissue folding and extension | A John, M Rauzi | MuVi SPIM | adherens junctions, tissue folding and extension, embryogenesis | Drosophila, In toto 4-D imaging, digital reconstruction, data processing, Drug injections, Dendra photoconversion, Cry2 optorecruitment, Actomyosin laser ablation |
| 2021 | ELife | Endogenous protein tagging in medaka using a simplified CRISPR/Cas9 knock-in approach | A Seleit, A Aulehla, A Paix | TruLive 3D Imager | technology Crispr/Cas9, protocol | Crispr/Cas9, medaka, whole-genome sequencing |
| 2021 | Nature | Smad4 controls signaling robustness and morphogenesis by differentially contributing to the Nodal and BMP pathways | L Guglielmi, C Heliot, S Kumar, Y Alexandrov… Caroline S. Hill | MuVi SPIM | morphogens, morphometry | TGF-β family, SMAD4, zebrafish, Nodal, zebrafish, image analysis |
| 2021 | Cell Reports | Endothelial Wnts control mammary epithelial patterning via fibroblast signaling | J Wang, W Song, R Yang, C Li, T Wu, XB Dong, B Zhou… Yi Arial Zeng | MuVi SPIM | endothelial cells, Wnt, fibroblasts | fluorescent mouse, organoid, FACS, immnohistochemistry, RNA-seq |
| 2020 | Semi-automatic generation of tight binary masks and non-convex isosurfaces for quantitative analysis of 3D biological samples | |||||
| 2020 | Front. Plant Sci. | Advanced Microscopy Reveals Complex Developmental and Subcellular Localization Patterns of ANNEXIN 1 in Arabidopsis | M Tichá, H Richter, M Ovečka, N Maghelli…Olga Šamajová | MuVi SPIM | Annexin 1, cell biology, lateral root growth | Arabidopsis, transgenic lines, immunoblotting, image analysis, Quantitative PCR |
| 2020 | Elife | Tracking cells in epithelial acini by light sheet microscopy reveals proximity effects in breast cancer initiation | A Alladin, L Chaible, L Garcia del Valle, R Sabine… Martin Jechlinger | InVi SPIM | cancer environment, oncogenes, breast cancer | mouse, 3D acinus cultures, qPCR analysis, image analysis, |
| 2020 | Cell Discovery | Light-sheet fluorescence imaging charts the gastrula origin of vascular endothelial cells in early zebrafish embryos | M Pang, L Bai, W Zong, X Wang, Y Bu, C Xiong… Jing-Wei Xiong | MuVi SPIM | cell lineage map, vascular endothelial cells | zebrafish, alignment, fusion, and extraction all-in-one software (AFEIO) for processing big data, quantitative analysis, photoconversion |
| 2020 | Macromolecular Biosciences | Poly(N‐isopropylacrylamide)‐Based Polymers as Additive for Rapid Generation of Spheroid via Hanging Drop Method | V RA, S Kumari, P Poddar, D Dhara… Souvik Maiti | MuVi SPIM | Multicellular tumor spheroid, protocol, tissue clearing | |
| 2020 | Journal of Biophotonics | Tissue clearing‐based method for unobstructed three‐dimensional imaging of mouse penis with subcellular resolution | P Matryba, A Wolny, M Pawłowska, ..Jakub Golab | tissue clearing | mouse penis | |
| 2020 | Elife | Mouse retinal cell behaviour in space and time using light sheet fluorescence microscopy | C Prahst, P Ashrafzadeh, T Mead, A Figueiredo…Katie Bentley | MuVi SPIM | retina, opthalmology, vasculature | mouse, image processing |
| 2020 | Current Biology | Cell death in cells overlying lateral root primordia facilitates organ growth in Arabidopsis | S Escamez, D André, B Sztojka, B Bollhöner, H Hall… Hannele Tuominen | - | lateral root growth, cell death, cell biology | Arabidopsis, Gravitational induction of LRP initiation, Transmission electron microscopy, Light microscopy, Laser-assisted targeted cell elimination, RNA isolation and qPCR |
| 2020 | Front. Bioeng. Biotechnol. | Opsef: Open source python framework for collaborative instance segmentation of bioimages | TM Rasse, R Hollandi, P Horvath | MuVi SPIM | Python framework for deep learning-based instance segmentation | Arabidopsis, image analysis |
| 2020 | Developmental Dynamics | Fusion of airways during avian lung development constitutes a novel mechanism for the formation of continuous lumena in multicellular epithelia | MA Palmer, CM Nelson | MuVi SPIM | avian lung development, epithelium | chicken, lung, photomanipulation |
| 2020 | ALTEX | Incorporation of stem cell-derived astrocytes into neuronal organoids to allow neuro-glial interactions in toxicological studies | M Brüll, AS Spreng, S Gutbier, D Loser, A Krebs… | MuVi SPIM | neuro-glial interactions , astrocytes, organoids | cell culture, proliferation, imaging |
| 2020 | Elife | Accurate and versatile 3D segmentation of plant tissues at cellular resolution | A Wolny, L Cerrone, A Vijayan, R Tofanelli, AV Barro… | MuVi SPIM | PlantSeg, cell segmentation | image analysis |
| 2020 | Current Biology | Axis specification in zebrafish is robust to cell mixing and reveals a regulation of pattern formation by morphogenesis | T Fulton, V Trivedi, A Attardi, K Anlas, C Dingare… | MuVi SPIM | morphogenesis, axis formation, development | zebrafish, explants,Mosaic Labeling, pharmacological inh, HCR, immunohistochemistry |
| 2020 | Developmental Cell | Tissue-scale mechanical coupling reduces morphogenetic noise to ensure precision during epithelial folding | AS Eritano, CL Bromley, AB Albero, L Schütz, FL Wen… | MuVi SPIM | development, epithelium, transcription | Drosophila, laser ablation, optogenetics, modelling |